Elucidating the growth performance genes in the Taiwan buffalos
Taiwan buffalo is the symbol of country landscape in Taiwan. Today, because the agricultural machinery is handy and popular, Taiwan buffalos do not need to work for farmland and so on their population is reduced. Therefore, based on the goal of Taiwan buffalo conservation, we want to understand the growth performance and the genetic background of this animal.
This study was conducted to elucidate the growth related gene expressions in the Taiwan buffalos. IGF-1, Leptin and Myostatin were target genes in this study. First of all, the serums from buffalos were harvested from the whole herd, and then ELISA was applied for determining the concentrations of IGF-1, Leptin and Myostatin.
In the other hand, the buffalo genomic DNA were extracted from the whole blood, and the gene specific primers were designed and applied for PCR to amplify the fragments which might alter in the individuals. After fragments sequenced, the DNA sequences will be made comparisons between the individuals. Ultimately, we want to understand the relationships among the buffalo growth genes, gene expressions and growth performances.
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