Effect of the glycerol concentration in extender on the quality of frozen-thawed semen of Formosan sambar deer

Hsin-Hung Lin, Chih-Hua Wang, Shann-Ren Kang, Wen-Lin Song, Chin-Hui Tseng, Mu-Jung Cheng, Shyh-Shyan Liu, and Perng-Chih Shen

　　The aim of this study was to examine the effect of the glycerol concentration in extender on the quality of frozen Formosan Sambar deer semen after thawing. Only the semen with sperm motility score (SMS) ≧4 was used. Fresh semen collected by electro-ejaculation was frozen with three different concentrations of glycerol (3%, 6%, 12%) in TES-yolk extender. Results showed that the sperm viability of the semen frozen in the extender containing 12% glycerol (45.9%) was significantly lower than that of the semen frozen in the extender containing 6% glycerol (63.1%). The sperm acrosomal integrity of the semen frozen in the extender containing 12% glycerol (48.1%) were significantly lower than those in 3% (63.3%) and 6% (68.7%) glycerol groups. However, the sperm motility of the semen frozen in the extender containing 6% glycerol (4.0) was significantly higher than those of other two treatments (both 3.3). In conclusion, the cryopreservation of Taiwan Sambar deer semen with TES-yolk extender supplemented with 6% glycerol was better for the sperm viability, acrosomal integrity and motility.